here is something similar on another cube. the branch to the right of the star is all solid dark blue.
Out of curiosity, is this an electrical synapse?
@malaclypse I believe that big swirly thing you found is a blood vessel, cool huh?
@rprentki About the little circles within the cells: I’m guessing those are vesicles stained by the small amount of dye that managed to get through, right? I was going to ask about that myself, as I noticed some of them in areas that one would expect to see vesicles, such as in axon terminals and where the golgi body would be in cell bodies.
Also, are you guys planning on using a stain that stains the proteins associated with electrical synapses (or otherwise allows one to more clearly locate them - I have no idea whether or not any such stains exist) at some point?
@malaclypse, it sounds like the small circles you found are probably vesicles. I’m not sure what out plans are for using different stains, that’d be a good question to ask Sebastian during the next google hangout though.
This seems like 2 different dendrites that got merged, the screens may not be clear, but I recorded the cube under all angles if needed :
IMAGE DECOMPRESSION FAILURE
@paps you’re right, that is definitely a merger. In case anyone is having trouble seeing that I’ve marked it below.
Ms. Prentki,
a merger error of AI on amacrine cell?
Here’s another example of a merger … confirmed by GrimReaper.
(You may have to open the files, I am lame at uploading images)
This is a really bad merger. I am going to stop mapping this block for now until it is cleared.
@marimacdonald and @jzezel you both found the same merger (albeit in different cubes). The GrimReaper took care of that merger just now.
@jzezl You just uploaded tiffs, which are fine just not supported by the forum as images. In the future jpeg, png, bmp etc. Would probably work better.