I have started four differend tasks, were there is a fail betwin slice number 18 and 19. Slice 1 to 18 is from set and slice 19 and down is from a nother set of slices.
Have anyone else tryed that
Jens
Yes, we are aware of the issue. Sometimes you will be able to find your neuron in some distant location than you expected; continue tracing the neuron in this case. But sometimes you will not be able to find any relation between two jumpy slices; then just stop and ignore what appears after the big change. This will go into the instruction as one of the advanced topics and we will try to exclude such tasks in the future.
They must have gone bad due to mechanical failures during image acquisition process. Roughly speaking, the images are acquired by a combo machine of slicer & microscope (called SBF-SEM; Serial Block-Face Scanning Electron Microscopy). The slicer cuts off the top of a block of retina tissue (in 22 nm thickness), and the microscope is synchronized to it and takes the image of every “new surface” of the block. So if the slicer fails and cuts off thicker slices, then we lose image between the slices and “jump” between the slices appears.
shall I press “Abort task” or “I´m finished” if I come to a big change?
Jens
You can abort the task if you don’t want to mess with it. It’s just fine.
But if you are willing to be a little more kind and helpful to us, carefully look at the coloring before and after the big change. The goal is to do as much as we can without introducing error:
1) If a colored piece hangs over the big change and the coloring does not seem right but you cannot deselect it, then abort the task
2) If the coloring stops before the change and the coloring seems correct within good slices, then press “I’m finished”.
Okay, I will try that.
Jens